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Dernière publication 01/09/2017

Primeras fases del desarrollo de un modelo experimental para el estudio de la re

Aim: To develop an animal model of chronic rotator cuff tear. To induce a chronic pattern similar to human being. Methods: A randomized with 40...

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    [title] => Primeras fases del desarrollo de un modelo experimental para el estudio de la re
    [paragraph] => Primeras fases del desarrollo de un modelo experimental para el estudio de la reparación de las roturas del manguito rotador
    [content] => 
Authors
G. Busto, A. Jauregui, R. Cuellar

 Lab
Instituto de Investigación Sanitaria Biodonostia. Donostia-San Sebastián, Gipuzkoa
Journal
ARTROSCOPIA Y CIRUGÍA ARTICULAR
Abstract
Aim:
To develop an animal model of chronic rotator cuff tear. To induce a chronic pattern similar to human being. 
Methods: 
 A randomized with 40 Wistar-Ham rats 200300 grams. Both forelimbs in 2 groups were involved: a) simple technique of tendon detachment (ST); b) technique in which suprascapular nerve denervation (DT) was added. At 4, 8 and 12 weeks were made macro- and microscopic study. The contralateral side served as control. 
 Results: 
 Both the TS and TD weight and volume produced supraspinatus (SE) and infraspinatus (IE) below the shoulder control. This effect was maintained over time. Muscle fatty infiltration increased over time and was higher at 12 weeks by TD in the muscles SE (p < 0.004) and IE (p < 0.02) than in controls. We found no significant differences in tendon parameters. 
Conclusions: 
 The changes in muscle histological properties were those of a chronic rotator cuff tear model reproducible and maintained over time. The effect of denervation in the group with the DT technique produced greater muscle fatty infiltration maintained over time highest ranking in the SE. We found no significant differences in the studied parameters tendon and was indifferent perform a technique or another as long as it was complete section of 2 or more rotator cuff tendons.
BIOSEB Instruments Used
Grip strength test (BIO-GS3)
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Une méthode simple pour quantifier objectivement la force musculaire des rats et souris et l'effet de drogues, toxines, maladies musculaires (ex: myopathie) et neurodégénératives. Cette mesure de force est souvent employée en association avec le test de coordination motrice ROTAROD: un sujet présentant une coordination normale montrera des résultats médiocres en cas de faible force musculaire. Un must pour vos recherches sur l'activité, la coordination et le contrôle musculaire: particulièrement utile pour vos études sur les maladies de Parkinson et Huntington.
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